Skip to main content
. 2007 Feb 23;189(9):3392–3402. doi: 10.1128/JB.01636-06

TABLE 3.

Summary of Ile-dependent growth assays with peptide transport mutantsa

Strain Growth (OD600) with peptidea
AI KI RI VI FI IS AK Trib Tetrab Hexab Nonab
WT 0.37 ± 0.04 0.37 ± 0.04 0.40 ± 0.04 0.42 ± 0.05 0.41 ± 0.04 0.38 ± 0.04 0.05 ± 0.02 0.41 ± 0.03 0.36 ± 0.02 0.36 ± 0.02 0.14 ± 0.01
oppA 0.39 ± 0.05c 0.40 ± 0.04c 0.38 ± 0.02c 0.42 ± 0.01c 0.37 ± 0.03c 0.42 ± 0.06c 0.05 ± 0.01c 0.38 ± 0.03c 0.36 ± 0.03c 0.36 ± 0.06c 0.14 ± 0.00c
oppB 0.22 ± 0.04 0.26 ± 0.04 0.18 ± 0.04 0.18 ± 0.04 0.30 ± 0.01 0.27 ± 0.03 0.05 ± 0.01c 0.26 ± 0.01 0.29 ± 0.01 0.38 ± 0.03c 0.22 ± 0.01
oppC 0.42 ± 0.02c 0.36 ± 0.04c 0.37 ± 0.04c 0.44 ± 0.03c 0.40 ± 0.04c 0.38 ± 0.04c 0.06 ± 0.00c 0.43 ± 0.04c 0.42 ± 0.03 0.38 ± 0.05c 0.19 ± 0.01
oppB oppC 0.27 ± 0.03 0.29 ± 0.01 0.29 ± 0.03 0.26 ± 0.02 0.26 ± 0.01 0.25 ± 0.03 0.07 ± 0.00c 0.24 ± 0.02 0.30 ± 0.01 0.35 ± 0.01c 0.22 ± 0.04
dppA 0.07 ± 0.02 0.25 ± 0.01 0.31 ± 0.01 0.14 ± 0.01 0.38 ± 0.03c 0.06 ± 0.01 0.04 ± 0.01c 0.25 ± 0.02 0.28 ± 0.01 0.06 ± 0.01 0.04 ± 0.01
dppB 0.10 ± 0.01 0.28 ± 0.02 0.27 ± 0.02 0.19 ± 0.01 0.35 ± 0.04 0.06 ± 0.01 0.04 ± 0.01c 0.33 ± 0.03 0.37 ± 0.03c 0.05 ± 0.01 0.04 ± 0.00
dppC 0.07 ± 0.01 0.22 ± 0.01 0.32 ± 0.02 0.14 ± 0.01 0.25 ± 0.02 0.05 ± 0.01 0.06 ± 0.01c 0.14 ± 0.04 0.34 ± 0.01 0.05 ± 0.00 0.05 ± 0.00
dppB dppC 0.06 ± 0.00 0.21 ± 0.03 0.32 ± 0.04 0.19 ± 0.02 0.22 ± 0.02 0.07 ± 0.01 0.06 ± 0.00c 0.10 ± 0.01 0.32 ± 0.03 0.06 ± 0.01 0.05 ± 0.00
dppA oppA 0.07 ± 0.01 0.27 ± 0.01 0.33 ± 0.02 0.14 ± 0.01 0.35 ± 0.01 0.07 ± 0.01 0.04 ± 0.01c 0.23 ± 0.02 0.24 ± 0.01 0.05 ± 0.00 0.05 ± 0.00
dppB oppB 0.06 ± 0.00 0.23 ± 0.02 0.16 ± 0.04 0.18 ± 0.01 0.13 ± 0.01 0.06 ± 0.00 0.07 ± 0.00c 0.25 ± 0.02 0.23 ± 0.01 0.06 ± 0.00 0.05 ± 0.00
a

Results shown are the average OD600 values for three independent assays with cells grown for 24 h in CDM supplemented with the indicated peptide as the sole Ile source. The initial culture OD600 was ∼0.05. Growth of the mutants was statistically significantly less than that of the parent strain (P < 0.05) unless indicated otherwise. Conditions under which no growth occurred are indicated in bold.

b

All amino acid sequences can be found in Table 2.

c

Growth data statistically indistinguishable from that for the wild type (P > 0.05).