TABLE 2.
Oligonucleotides used for plasmid and strain construction
| Function and name | Oligonucleotide sequence (5′-3′)a |
|---|---|
| Construction of plasmids | |
| cbpAfor | TAATGAATTCACCATGGAATTAAAGGATTATTACGCC |
| cbpArev | CATTAAGCTTTTATGCTTTCCCCCAATCTTT |
| cbpMfor | CCATCCATGGCTAATGTTACGGTGACTTTT |
| cbpMrev | AGTTAAGCTTTCACGGATGAGCTACAAACCG |
| Chromosomal mutations | |
| ΔcbpAfor | GGGGTTCCTTCAATTTGTGTTGATTTACGCGAGATAACGCTTGTGACGGAAGATCACTTCGC |
| ΔcbpArev | CGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCTTACGCCCCGCCCTGCCACTCATCG |
| ΔcbpMfor | CCAGTCGTCTTTTGATCCACGTAAAGATTGGGGGAAAGCATAATGTGACGGAAGATCACTTCGC |
| ΔcbpMrev | GTAGACCGGTTAAGATGCGTCATCGCATCCGGCAAACACACATTACGCCCCGCCCTGCCACTCATCG |
a
Underlined bases indicate regions of homology with DNA in or near cbpA or cbpM.