TABLE 2.
SadB-dependent biofilm and swarming phenotypes of Che cluster mutants
| Chemotaxis cluster | Mutation | Swarming phenotype | SadB biofilm stimulationc,d | SadB swarm repressionc,e |
|---|---|---|---|---|
| ΔmotABa | WT | N | N | |
| I | ΔmotCD | Nonswarming | N | ND |
| II | PAO178::pKO3PAO178 | Decreased | Y | Y |
| III | wspA::pKO3wspA | WT | Y | Y |
| IV | pilG::Tn5B21 | Hyperswarmerb | N | M |
| IV | ΔpilJ | Hyperswarmerb | N | N |
| IV | ΔchpB | Nonswarming | ND | ND |
| V | cheR1::pKO3cheR1 | Decreased | Y | Y |
a
These genes are not in clusters CheI to CheV.
b
As determined by a lack of avoidance of other bacterial swarms, as reported elsewhere (3).
c
N, no; Y, yes; M, moderate; ND, not determined.
d
Determined by testing whether SadB expressed from a plasmid stimulated biofilm formation (see Fig. 1A).
e
Determined by testing whether SadB expressed from a plasmid repressed swarming motility on 0.55% agar (see Fig. 1D).