TABLE 2.

Plasmids and constructs

Plasmid	Description	Reference
pUC18	E. coli multicopy plasmid	49
pUSRT3-1	pUC18 with a 3.9-kb SacI/PstI fragment harboring tuf3	44
pUSRT3-3	pUC18 with 2.8-kb fragment harboring tuf3, preceded by an introduced EcoRI site and an 0.2-kb tuf1 upstream coding sequence	44
pUSRT3-6	pUC18 with 2.8-kb EcoRI/PstI fragment harboring tuf3, lacking any upstream coding sequence	This work
pIJ4070	pUC18 with 0.3-kb fragment harboring the ermE promoter region	M. J. Bibb, unpublished data
pUSRT3-7	pUC18 with 2.8-kb EcoRI/PstI fragment harboring tuf3, preceded by an 0.3-kb fragment harboring the ermE promoter region	This work
pIJ487	Streptomyces multicopy plasmid	45
pIJ4083	pIJ487 with DNA fragment harboring Pseudomonas putida xylE, lacking any upstream coding sequence	7
pISRT3xylE-1	pIJ4083 with DNA fragment harboring the tuf3 upstream region	This work
pISRT3-1	pIJ487 with 3.1-kb KpnI/PstI fragment from pUSRT3-7, harboring tuf3, preceded by an 0.3-kb fragment harboring the ermE promoter region	This work