TABLE 3.

Sequences of the gene-specific primers used in quantitative RT-PCR assays for Synechococcus strain PCC 7002^a

Clone	GenBank accession no.	Forward primer (5′-3′)	Reverse primer (5′-3′)
ndhF3	U97516	ACCGCGATGTATCTTTATCTC	ACAAAATAGCGGTCTAACCAG
ndhD3	U97516	CGTGTTCCAAGGTAGCTTTAG	AAAAGGATAATGACCGTCAAG
chpY	U97516	AAATTATTCCCAAATCCAAAG	GCATACAAAATACCGTAGTGG
ORF133	U97516	TGCCAAGTATTGTGGATATTG	TAAGTGAGAATACGCGCTAAC
ORF133+	U97516	CGCGTATTCTCACTTATCACG	TGAAACCGGAAAATTTGAAAC
sbtA	DQ632590	CAGCGATTGTTGTAGCTAGTC	AATTTCAACCCGATTAGTAGC
porB	DQ632588	TTACAAGCCTACAATTTTTCG	CCGTTAATTTATCTGTGATCG
bicA	AF381039	GTTCTTTTGTTTCAACTCAGTG	GCTTAAAGAGCTTGAGTTTTTC
nhaS3	AF381039	GATTTCGTTCCTTCCCTGTC	GAGACCCCGACTAGAACACC
mnhC	AF381039	ATCGCGTATTACATTCTCGTG	ATTATCCCGTGACAACTTCATC
mnhD1	AF381039	GGGATAATCCGACCTTAGAAAC	TCCAAGAGTTCTAGGGTAAAAGG
mnhD2	AF381039	AGAGCGGCTATTTTATTTTGAC	AACGTAGAGGCTAATCAGATCG
HCP 1	AF381039	GATTAAAGATTGGCTCCATGTG	ACAAAATTAGGACACCGACAAC
HCP 2	AF381039	GCGTTACTACTCCCGATTTATG	ACAAAATTAGGACACCGACAAC
HCP 3	AF381039	CGTTCTGTGTTGTTTAAATTGC	CACATACCCCAGTACCGTATTC
HCP 4	AF381039	CCTTGTTATTGCCCGTGAC	AACATCGTCCCCACTAACG
mnhB	AF381039	CTTTATTTGTGAAAATGCTCGTC	GGGAATCGGTAAATTGCTTAAC
mnhB+	AF381039	TGCCATTTATAAAAAGTGGAATG	CAAACCGTATGCTGGTATGAC
rbcS	AF015889	TGATGGATCAAGGCTATATTCC	AACAACACGGATGTAGCAGTC
rbcX	AF015889	AACAACACGGATGTAGCAGTC	ACTCACCAGGGTTAGGTTCAG
rbcL	AF015889	TACTTGGACCACTGTATGGAC	TCAAAACGTTGGTTACAGAAC
ccmK1	AF015889	CTATTGCAGTCGGAATGGTAG	GGAAGCTTGGACTTCAGAAAC
ccmK2	AF015889	CGTGGTGATGTTTCTGAAGTC	ACTTCTTCGGTGTAGCGAATC
ccmL	AF015889	CAGCACCTACAAAGCAGAAAG	GTATCAATGATGCCAATGACC
ccmM	AF015889	TCGAATTTAATTGGTGATGTC	ATCCAGACAGAATAGTCATGG
ccmN	AF015889	AAATGCCTGCTTAGGTTATGG	TTGTGGCAGTCTGGTTAGTTG
ccmO	To be assigned	ACAACTGGCCTCCTATGAAAC	TTGGTTCTGCCTTATCTAGCC
ccaA	To be assigned	TATGCCTTTGGTTTACGATTG	ATCCAGCCATAGATTTTCAGC
p450	U97516	CTGACCCGATGGACATTTAC	AGCAGATAAATTGACCCCATC

^aHCP, hypothetical conserved protein from the bicA operon (nomenclature as per Fig. 5).