TABLE 5.
Amplification of mRNA sequences from the ccmR region of Synechococcus strain PCC 7002 by RT-PCRa
| Primer pair coordinates | Junction | Product | Primer pair coordinates | Junction | Productb | |
|---|---|---|---|---|---|---|
| 1350F-2002R | Upstream/ccmR | N | 4228F-4670R | ndhF3/ndhD3 | Y | |
| 1521F-2002R | ccmR/ndhF3 | Y | 5806F-6253R | ndhD3/chpY | Y | |
| 1521F-2876R | ccmR/ndhF3 | Y | 7172F-7602R | chpY/ORF133 | Y | |
| 1521F-3215R | ccmR/ndhF3 | N | 7589F-7963R | ORF133/downstream | Y* | |
| 1521F-4670R | Upstream/F3 | N | 7589F-8288R | ORF133/downstream | Y* | |
| 1950F-4670R | Upstream/F3 | Y | 82737F-8726R | ORF133/downstream | Weak* |
a
The sequence map coordinates of primers (according to Fig. 4; GenBank accession no. U97516) and the presence (Y) or absence (N) of an amplified product are indicated. First-strand cDNA template was generated from RNA derived from Synechococcus strain PCC 7002 cells transferred from 2% CO2 to air bubbling for 30 min.
b
*, Not Ci responsive.