TABLE 6.
Amplification of mRNA sequences from the bicA region of Synechococcus strain PCC 7002 by RT-PCRa
| Primer pair coordinates | Junction | Product | Primer pair coordinates | Region or junction | Product | |
|---|---|---|---|---|---|---|
| 1279F-1980R | Upstream/bicA | N | 8014F-8486R | ORF5/6 | Y | |
| 1462F-1980R | Upstream/bicA | Y | 8333F-8774R | ORF6/7 | Y | |
| 1561F-1980R | Upstream/bicA | Y | 8613F-9081R | ORF7/8 | Y | |
| 3198F-3700R | bicA/napA | Y | 8923F-9325R | ORF8/9 | Y | |
| 4697F-5247R | napA/ORF3 | Y | 9510F-9976R | ORF9/10 | Y* | |
| 5061F-5462R | ORF3/4 | Y | 10140F-10539R | ORF10/downstream | Y* | |
| 6718F-7128R | ORF4/5 | Y | 10181F-10692R | ORF10/downstream | Med* |
a
The sequence map coordinates of primers (according to Fig. 5A; GenBank accession no. AF381039) and the presence (Y) of an amplified product are indicated. First-strand cDNA template was generated from RNA derived from Synechococcus strain PCC 7002 cells transferred from 2% CO2 to air bubbling for 30 min. *, Not Ci responsive.