FIG. 6.

Mode of nuclease activity of recombinant GST-mhp379. Approximately 4 μg of recombinant GST-mhp379 was incubated with PCR-amplified 5′ DIG-labeled DNA substrate. The reaction products were analyzed by 1% agarose gel electrophoresis with molecular mass markers (HindIII-digested phage λ DNA), and the DNA was visualized by staining with ethidium bromide. The DIG-labeled reaction products were detected by Southern immunoblotting of the agarose gel. An aliquot of each reaction mixture was analyzed at different times, as indicated for each lane. The nuclease activity of an equimolar concentration of GST was analyzed in parallel (GST control).