TABLE 2.
Primers used in this study
| Primera | Sequenceb | Descriptionc |
|---|---|---|
| Cps2-D2 (+) | GGTTCTTATGGAGATTACGGGAA | cps2D4996-5018 |
| Cps2-D3 (+) | CTCACAGGCAAAATTGGATTTTG | cps2D4368-4390 |
| Cps2-E10 (+) | ATTTACTTCCTCACATTACATG | cps2E5343-5364 |
| Cps2-E11 (−) | AAACTACTTCGCTCCATCTCTC | cps2E6418-6396 |
| Cps2-T1 (−) | CTCATGACCATCTGGATTTAC | cps2T6449-6468 |
| Cps2-T2 (+) | TTATATCATTGGTTCAAAGGGG | cps2T6459-6481 |
| Cps2-G0 (+) | CAAGGACATGATGTGGTTTGTTA | cps2G8665-8688 |
| Cps2-G2 (−) | *CATTATAACTATCCATACTAATAA | cps2G9671-9647 |
| Cps2-I3 (−) | CACCTGAATTTGTCCCAATAAC | cps2I11906-11884 |
| Cps2-I2 (+) | *TAAAAATGGATGGGGAAATTCAA | cps2I10830-10854 |
| Cps2-I4 (+) | TTCGATAGTTGAGGATTCAGACTTT | cps2I11039-11065 |
| Cps2-J1 (+) | CTTGTAGTAAAATACTTGCTAAG | cps2J13034-13058 |
| Cps2-J12303F (+) | TTCTGAAGGGGTTCTTCGATTTGCA | cps2J12303-12327 |
| Cps2-J2 (−) | *CATTTTTCTCCTTTCAATACTCGT | cps2J13561-13527 |
| Cps2-J7 (+) | *TAAGAACCAATAAGTACGAGTAT | cps2K13522-13544 |
| Cps2-J8 (−) | *CAATTTTCTAGTTCCTTATATAGT | cps2J12116-12094 |
| Cps2-K1(−) | AACTACTCTTACTCCCTTAGCTTTTA | cps2K14627-14602 |
| Cps2KORF-BglII (+) | **AAAATAGCAGTAGCAGG | cps2K13564-13581 |
| Cps2KORF-KpnI (−) | *TTAATCTCTTTCAAAAATA | cps2K14798-14780 |
| Cps2-P1 (+) | *GAAAGAGATTAATTTAGTATATT | cps2P14787-14809 |
| Cps2-P15468R (−) | CTTCCTCTACTACACTAAGTATCC | cps2P15468-15445 |
| Cps2-P2 (−) | CATGCGTTATGACTGTCTTAGG | cps2P15283-15262 |
| Cps3D-F (+) | *GAGGACTGTAGTAAAAT | cps3D1012-1029 |
| Cps3D-R (−) | *CCCTTATTCTCTGCC | cps3D2215-2210 |
| CpsL-1 (+) | AGGTTATTTCATTATGAAAGG | cps2L15496-15517 |
| CpsL-2 (−) | CCGAAAAAATTATCTGTCATCTAG | cps2L16399-16375 |
| c-ups1 | GAGCCCATGTTTCTCAATAGG | cps2449-470 |
| c-ups2 | ATCTTAGTAGACTTCCCGCG | cps21373-1352 |
| KM151-2 (−) | *GTACTAAAACAATTCATCCA | aphA-32543-2523 |
| KM151-3 (+) | *GAGGAAGGAAATAATAA | aphA-31729-1746 |
| LDH-F (+) | GTCGGTGATGGTGCTGTAGGTTCATC | ldh164-189 |
| LDH-R (−) | GTCGATGTTAGCGTGTGACCAAACAG | ldh710-687 |
| Ugd-1 (+) | GGGCATTCTTCCATCTAAAAATGA | ugd148064-148088 |
| Ugd-2 (−) | GCATTTAAACTTCTCCTCTCAGC | ugd148627-148604 |
a
Forward and reverse primers are indicated by + and −, respectively.
b
* and **, KpnI (GGTACC) and BglII (AGATCT) sites present at the 5′ end.
c
Superscript numbers indicate the positions of the primer start and end in the homologous sequence of either type 2 capsule (cps2) (GenBank accession no. AF026471), type 3 capsule (cps3) (GenBank accession no. U15171), pDL276 aphA-3 (GenBank accession no. AF216803), ldh (TIGR4 sequence) (GenBank accession no. AE007422), or R6 ugd (GenBank accession no. NC003098).