Table 1. Sequence profiles of TaqI-RE-digestion-resistant mutant SDHB clones.

Nucleic acid source (n = number of samples)	Nucleic acid type/PCR method	No. of clones TTGA R46X	No. of clones TCGG	No. of clones TCAA	No. of clones CCGA	No. of clones ACGA	No. of clones TAGA	No. of clones TCGT	Single clones	Total no. of clones	P for enrichment of TTGA clones&
PBMC (n = 6)	cDNA/RT-PCR	92	0	0	0	0	0	0	TTGG	93	∼0
Pure Monocytes (n = 1)	cDNA/RT-PCR	32	2	0	2	0	0	0	TGGA	37	∼0
T-ALL primary (n = 3)	cDNA/RT-PCR	75	13	4	5	0	0	0	TGGA	98	∼0
T-ALL cell lines*	cDNA/RT-ePCR	27	7	6	9	3	0	1	ATAG	54	2.2×10−4
NK-92 (NK cell)	cDNA/RT-ePCR	2	1	2	10	8	0	3	0	26	0.33
Pfeiffer (B cell)	cDNA/RT-ePCR	2	2	0	16	0	0	3	0	23	0.33
THP-1 (Monocyte)	cDNA/RT-ePCR	9	10	13	14	4	3	1	TCACTCTA	56	0.60
PBMC (n = 3)	gDNA/ePCR	27	0	0	0	0	2	0	TGGA	30	∼0
T-ALL cell linesˆ	gDNA/ePCR	43	0	1	3	0	6	0	TCTA	54	∼0

^*Includes Jurkat, Loucy, SUP-T1, HuT 78.

^ˆIncludes MOLT-4 and Jurkat.

^&Fisher's exact test of the hypotheses that number of TTGA clones should be ∼16% of the total on the basis of random somatic mutation data [15].