Figure 5 Effect of tumour necrosis factor receptor (TNFR) deficiency on activation of Kupffer cells during methionine and choline deficient (MCD) diet induced steatohepatitis. (A) Immunohistochemical detection of F4/80 positive cells in the liver. To identify the infiltrating cell types, we performed immunohistochemical analysis using a monoclonal antibody specific for F4/80 antigen, a surface marker of mouse monocytes/macrophages and Kupffer cells. Representative liver samples derived from control diet fed +/+ (Cont; +/+), control diet fed −/− (Cont; −/−), MCD diet fed +/+ (MCD; +/+), and MCD diet fed −/− (MCD; −/−) mice (×100). B: Hepatic CD14 expression levels. Results (mean (SEM)) of five mice/group at the end of the feeding period are shown. All real time quantitative polymerase chain reactions were performed in duplicate. *p<0.05 compared with the other groups; †p<0.05 compared with the control group. Control, mice pair fed an isocaloric liquid control diet; MCD, mice pair fed an isocaloric liquid MCD diet.