Figure 6 Effect of tumour necrosis factor receptor (TNFR) deficiency on activation of Kupffer cells during methionine and choline deficient (MCD) diet induced steatohepatitis. (A) Hepatic intracellular adhesion molecule 1 (ICAM‐1), (B) vascular cell adhesion molecule 1 (VCAM‐1), and (C) tumour necrosis factor α (TNF‐α) expression levels. Real time polymerase chain reaction analysis quantitated hepatic mRNA levels of ICAM‐1, VCAM‐1, and TNF‐α in liver homogenates from each group. Results (mean (SEM)) of five mice/group at the end of the feeding period are shown. All real time quantitative polymerase chain reactions were performed in duplicate. *p<0.05 compared with the other groups; †p<0.05 compared with the control group. Control, mice pair fed an isocaloric liquid control diet; MCD, mice pair fed an isocaloric liquid MCD diet.