Figure 5 Gene expression of interleukin (IL)‐1β, IL‐6, interferon γ (IFN‐γ), tumour necrosis factor α (TNF‐α), Toll‐like receptor 2 (TLR‐2) and 4 (TLR‐4), and cyclooxygenase 2 (COX‐2) in the stomach of wild‐type (WT) and Aα^0/0 mice at six months old, as detected using reverse transcription‐polymerase chain reaction (PCR). PCR products were separated on 2.0% agarose gels and stained with ethidium bromide. Gene expression of IL‐1β, IFN‐γ, TLR‐2, TLR‐4, and COX‐2 was upregulated in Aα^0/0 mice compared with WT mice. There was no significant difference in gene expression of IL‐6 and TNF‐α between WT mice and Aα^0/0 mice.