Table 1 Antibodies for immunohistochemistry used in this study.
| Primary antibody | Secondary antibody | ||||||
|---|---|---|---|---|---|---|---|
| Name | Company | Dilution | Incubation | Name | Company | Dilution | Incubation |
| Mouse anti‐αSMA | Sigma | 1:400 | 1 h RT | Goat anti‐mIgG‐HRP | Sigma | 1:100 | 30 min |
| Mouse antidesmin | Sigma | 1:20 | 1 h RT | Goat anti‐mIgG‐HRP | Sigma | 1:100 | 30 min |
| Mouse antivimentin | Chemicon | 1:200 | 1 h RT | Rabbit anti‐mIgM | MBL | 1:300 | 30 min |
| Followed by Envision | Dako | – | 30 min | ||||
| Rabbit anti‐col I | RDI | 1:400 | 1 h RT | Envision | Dako | – | 30 min |
| Rabbit anti‐col III | RDI | 1:500 | 18 h 4°C | Envision | Dako | – | 30 min |
| Rabbit anti‐CD3 | Dako | 1:500 | 1 h RT | Envision | Dako | – | 30 min |
| Rat antimouse F4/80 | Serotec | 1:100 | 18 h 4°C | Goat anti‐rIgG‐biotin | Santa Cruz | 1:200 | 1 h |
| Streptoavidin‐HRP | Dako | 1:300 | 30 min | ||||
| Goat anti‐mMCP‐1 | Santa Cruz | 1:50 | 18 h 4°C | Mouse anti‐g/s IgG‐HRP | Sigma | 1:100 | 1 h |
RT, room temperature; col, collagen; HRP, horseradish peroxidase; g, goat; s, sheep; m, mouse; r, rat; Envision, HRP coupled antirabbit secondary reagent.