Figure 7 Sulfasalazine partially reversed glycochenodeoxycholic acid (GCDCA) induced cholestasis. Dimethyl sulfoxide (0.1% v/v), GCDCA, sulfasalazine (SSZ), and GCDCA in combination with SSZ were administered for 60 minutes (GCDCA) and 70 minutes (SSZ). GCDCA significantly reduced bile flow (p<0.01 v control). While SSZ alone decreased bile flow, SSZ significantly increased bile flow in GCDCA treated livers (*p<0.05, **p<0.01 v control; †p<0.05 v GCDCA). Results are expressed as mean (SD) of six experiments.