Table 1 Sulfasalazine reduced glycochenodeoxycholic acid (GCDCA) induced oxidative stress in HepG2‐Ntcp cells.
| Condition | Fold ROS formation |
|---|---|
| Control | 1 |
| GCDCA 75 µmol/l | 1.28 (0.05)* |
| Sulfasalazine 1000 µmol/l | 0.97 (0.02) |
| GCDCA 75 µmol/l+sulfasalazine 1 µmol/l | 1.28 (0.04) |
| GCDCA 75 µmol/l+sulfasalazine 10 µmol/l | 1.24 (0.01) |
| GCDCA 75 µmol/l+sulfasalazine 100 µmol/l | 1.09 (0.03)† |
| GCDCA 75 µmol/l+sulfasalazine 1000 µmol/l | 1.00 (0.03)†† |
HepG2‐Ntcp cells were loaded for four hours with the fluorescent dye carboxy‐H2‐DCFDA (4 µmol/l) to detect generation of reactive oxygen species (ROS). Then, cells were incubated with GCDCA in the absence or presence of sulfasalazine for two hours.
Data represent the n‐fold increase in carboxy‐H2‐DCFDA fluorescence compared with controls (set at 1).
Results are the mean (SD) of 12 independent experiments.
*p<0.01 v control; †p<0.05, ††p<0.01 v GCDCA.