Table 1 Primers and polymerase chain reaction conditions for E‐cadherin, DAPK, MGMT, hMLH1, p16, and OR beta.

Locus	Allele	Sense primers	Antisense primers	Annealing temp (°C)
E‐cadherin	Methylated	5′′‐ TTAGGTTAGAGGGTTATCGCGT ‐3′	5′‐ TAACTAAAAATTCACCTACCGAC‐3′	57
	Unmethylated	5′‐ TAATTTTAGGTTAGAGGGTTATTGT‐3′	5′‐ CACAACCAATCAACAACACA‐3′	53
DAPK	Methylated	5′‐ GGATAGTCGGATCGAGTTAACGTC ‐3′	5′‐ CCCTCCCAAACGCCGA ‐3′	60
	Unmethylated	5′‐GGAGGATAGTTGGATTGAGTTAATGTT ‐3′	5′‐ CAAATCCCTCCCAAACACCAA ‐3′	60
MGMT	Methylated	5′‐ TTTCGACGTTCGTAGGTTTTCGC ‐3′	5′‐ GCACTCTTCCGAAAACGAAACG ‐3′	59
	Unmethylated	5′‐TTTGTGTTTTGATGTTTGTAGGTTTTTGT‐3′	5′‐AACTCCACACTCTTCCAAAAACAAAACA‐3′	59
hMLH1	Methylated	5′‐ TATATCGTTCGTAGTATTCGTGT ‐3′	5′‐ TCCGACCCGAATAAACCCAA‐3′	60
	Unmethylated	5′‐ TTTTGATGTAGATGTTTTATTAGGGTTGT ‐3′	5′‐ ACCACCTCATCATAACTACCCACA ‐3′	60
p16	Methylated	5′‐TTATTAGAGGGTGGGGCGGATCGC‐3′	5′‐GACCCCGAACCGCGACCGTAA‐3′	65
	Unmethylated	5′‐TTATTAGAGGGTGGGGTGGATTGT‐3′	5′‐CAACCCCAAACCACAACCATAA‐3′	60
OR beta	Methylated	5′‐TTTGGAAGGTGGGTTTGGTC‐3′	5′‐CGCATACAAATATAATAACTAACG‐3′	49
	Unmethylated	5′‐TTTGGAAGGTGGGTTTGGTT‐3′	5′‐CACATACAAATATAATAACTAACA‐3′	45

DAPK, DAP kinase; MGMT, O⁶‐methyl‐guanine methyltransferase; hMLH1, human Mut L homologue; OR beta, oestrogen receptor beta.