Table 2 Amino acid substitutions resulting in antagonists (p<0.02).
| Substitution | Antagonism (%) [p value] | Agonism (%) | Substitution | Antagonism (%) [p value] | Agonism (%) |
|---|---|---|---|---|---|
| 63Q→W | 14 (3) [0.001] | 38 (7) | 67P→A | 14 (5) [0.01] | 29 (5) |
| 64P→F | 14 (4) [0.006] | 8 (4) | 67P→D | 18 (4) [0.001] | 25 (10) |
| 64P→G | 15 (5) [0.01] | 18 (4) | 67P→G | 12 (3) [0.002] | 13 (3) |
| 64P→R | 18 (5) [0.002] | 18 (14) | 67P→H | 14 (5) [0.01] | 15 (6) |
| 64P→S | 16 (3) [0.0004] | 32 (7) | 67P→I‡ | 24 (6) [0.001] | 24 (7) |
| 64P→T* | 28 (4) [0.0001] | 47 (6) | 67P→K | 14 (6) [0.02] | 3 (2) |
| 64P→W† | 21 (6) [0.004] | 10 (6) | 67P→V* | 24 (4) [0.0001] | 30 (7) |
| 64P→Y† | 17 (7) [0.01] | 29 (11) | 68Y→E | 15 (6) [0.02] | 33 (5) |
| 65E→H† | 18 (6) [0.01] | 40 (6) | 68Y→L | 12 (4) [0.004] | 43 (5) |
| 65E→I‡ | 20 (5) [0.001] | 19 (5) | 68Y→S | 16 (5) [0.004] | 58 (9) |
| 65E→Q | 13 (5) [0.02] | 12 (2) | 69P→G | 13 (5) [0.01] | 50 (6) |
| 65E→R‡ | 16 (6) [0.02] | 7 (6) | 69P→I‡ | 10 (4) [0.01] | 57 (22) |
| 65E→S | 15 (3) [0.001] | 48 (5) | 69P→L | 17 (7) [0.02] | 31 (3) |
| 65E→T*† | 28 (5) [0.0001] | 3 (5) | 69P→N | 14 (6) [0.02] | 46 (10) |
| 65E→W | 17 (6) [0.01] | 8 (4) | 69P→Q | 12 (4) [0.01] | 35 (5) |
| 66L→G | 11 (4) [0.01] | 6 (4) |
Values are mean (SEM).
*Significant at p<0.02 after Bonferroni correction for multiple comparisons (131).
†Antagonism significant (p⩽0.005) when assessed in a second sample of 10 CD subjects with coeliac disease (CD).
‡Antagonism not significant (p>0.05) when assessed in a second sample of 10 CD subjects.