Table 1 Primer and probe sequences for real time‐polymerase chain reaction.

Gene	Forward	Reverse	Probe
SOCS‐3	5′‐CCCTCGCCACCTACTGAA‐3′	5′‐TCCGACAGAGATGCTGAAGA‐3′	NA
IFN‐R1	5′‐GTGGAACAGGAGCGATGAGT‐3′	5′‐CAACCTCATACCATGAAGAAGTG‐3′	NA
TNF‐α	5′‐CCCCAGGGACCTCTCTCTAA‐3′	5′‐CAGCTTGAGGGTTTGCTACA‐3′	5′‐AGCCCTCTGGCCCAGGCAGT‐3′
PEPCK	5′‐AGCTGGCAACATGGAGTCTT‐3′	5′‐CTTCCGGAACCAGTTGACAT‐3′	5′‐CCCTTCTTTGGCTACAACTTCGGCA‐3′
GAPDH	5′‐TGCACCACCAACTGCTTAGC‐3	5′‐GGCATGGACTGTGGTCATGAG‐3′	5′‐CCTGGCCAAGGTCATCCATGACAACTT‐3′

SOCS, suppressor of cytokine signalling; IFN‐R1, interferon receptor 1; TNF‐α, tumour necrosis factor α; PEPCK, phosphoenolpyruvate carboxy kinase; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NA, not applicable.