Figure 6 Connective tissue growth factor module 4 (CCN2₄) induces integrin α₅β₁ dependent pancreatic stellate cell (PSC) migration. (A) PSC migration assays were performed by placing the cells in culture inserts (2.5×10⁵ cells/insert) followed by incubation in a 12 well companion plate for six hours in the absence or presence of desired concentrations of CCN2₄ in the lower chamber. (B) PSC migration assays were performed in the presence of 100 ng/ml of CCN2_1–4, CCN2_3–4, CCN2₄, or 100 ng/ml fibronectin (FN) in the lower chamber. (C) Cell migration assays were performed following 30 minutes of preincubation of PSC with P5 (35 μM), anti‐α₅β₁ (25 μg/ml), mouse IgG (25 μg/ml), or heparin (2 μg/ml). Data are means (SD) of quadruplicate determinations and are representative of three experiments. **p<0.01 versus control; ††p<0.01 versus the “no add” group.