Table 1 Polymerase chain reaction (PCR) primers used in the study.

SNP	PCR primer sequences	Annealing temp (°C)
	Primers used for allele specific PCR
CTLA‐4 +49A/G	5′ CAAGCCAGATTGGAGTTTA 3′	T1 = 64
	5′ CTCAGCTGAACCTGGCTA/G 3′	T2 = 58
CTLA‐4 +6230G/A	5′ AGGAAGGCAGATCAAAATGC 3′	T1 = 65
	5′ CACCACTATTTGGGATATAACA/G 3′	T2 = 59
HGH	5′ GCCTTCCCAACCATTCCCTTA 3′
	5′ TCACGGATTTCTGTTGTGTTTC 3′
	Primers used for PCR‐RFLP
CTLA‐4 +49A/G	5′ CCACGGCTTCCTTTCTCGTA 3′	56
	5′ AGTCTCACTCACCTTTGCAG 3′
CTLA‐4 +6230G/A	5′ CATCCTTGCATTTGAATATTGTG 3′	59
	5′ AAGAATCACAGAGGGCCCAGTG 3′

CTLA‐4, cytotoxic T lymphocyte antigen 4; SNP, single nucleotide polymorphism; HGH, human growth hormone; RFLP, restriction fragment length polymorphism.