Figure 1 [−215G>A; IVS3+2] mutation produced a truncated transcript. Total RNA was isolated from the biopsy specimen of the stomach, and the entire coding region of the serine protease inhibitor Kazal type 1 (SPINK1) gene was amplified by reverse transcription polymerase chain reaction, followed by 2 % agarose gel electrophoresis. Sm, size marker (100 base pair ladders), WT, healthy control. Patient A with alcoholic chronic pancreatitis (CP) was homozygous for the [−215G>A; IVS3+2T>C] mutation. His daughter B and patient C with idiopathic CP were heterozygous. In subjects carrying the [−215G>A; IVS3+2T>C] mutation, two bands were observed: a fragment corresponding to a normal (“F1”) and a truncated (“F2”) band.