Table 1 Probes and primers used for real time polymerase chain reaction.

	Probe (5′FAM 3′TAMRA)	Forward primer, 5′‐3′	Reverse primer, 5′‐3′
SLC11A2 (DMT1) IRE+ve	CTC TAT CAG GCT TAG GAT TCT TTG AAC TTA TTT CCA CTT T	CCA TAT GAA ATA TAA AAT GAA GAG ACA CCT A	CCC CTC TTA ACT TCC ACT GAG AAA
SLC11A2 IRE−ve	CCC ACC CAT AAC AGT CAT ACA CTC CCA GAG T	TGG GAA GGG TGT TTC AAA ACT G	CCA TCA GAG GCC AAT CGT TTA
SLC40A1 (FPN)	AGGATTGACCAGTTAACCAACATCT TAGCCCC	AGC AAA TAT GAA TGC CAC AAT ACG	CAA ATG TCA TAA TCT GGC CAA CAG
Ferritin	CCA ACG AGG TGG CCG AAT CTT CCT T	GGA ACA TGC TGA GAA ACT GAT GAA	CAT CAC AGT CTG GTT TCT TGA TAT CC
HEPH	ACA GTG ACA TAG TGG CTT CCA GCT TCT TAA AGT CTG	GGA AGA AAT GTC ATC ACG AAC CA	TCC CCC TAT CCG GTT CTT G
TFRC (TFR1)	AAA GAC AGC GCT CAA AAC TCG GTG ATC ATA G	CGT GAT CAA CAT TTT GTT AAG ATT CA	CCA CAT AAC CCC CAG GAT TCT
CYBRD1 (DCYTB)	CCA GGG CAT CGC CAT CAT CGT	CATGGTCACCGGCTTCGT	CAG GTC CAC GGC AGT CTG TA
CDX‐2	CGA GCT AGC CGC CAC GCT GG	CTA CAT CAC CAT CCG GAG GAA	CAG ATT TTA ACC TGC CTC TCA GAG A
E‐cadherin	AAA TTC ACT CTG CCC AGG ACG CGG	GGC GCC ACC TCG AGA GA	TGT CGA CCG GTG CAA TCT T