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Figure 4 Analysis of the activities of various NS3/4A proteases by an in vitro transcription and translation assay. Protease activity was tested by an in vitro transcription and translation assay of NS3/4A genes with wild type (wt) or mutant 1073–1081 epitope sequences. The upper band corresponds to the non‐cleaved NS3/4A fusion protein and the lower band corresponds to the free NS3 protein. A single NS3/4A fusion band represents a non‐functional protease.
