Table 1.
Plasmid sets used for the recovery of A/WSN/33 (H1N1) and A/Teal/HK/W312/97 (H6N1) viruses
| Segment | A/WSN/33 (H1N1) | A/Teal/HK/W312/97 (H6N1) | ||
|---|---|---|---|---|
| 1 | pHW181-PB2 | pHW181-PB2 | pHW241-PB2 | pHW241-PB2 |
| 2 | — | pHW182-PB1 | — | pHW242-PB1 |
| 3 | pHW183-PA | pHW183-PA | pHW243-PA | pHW243-PA |
| 4 | pHW184-HA | pHW184-HA | pHW244-HA | pHW244-HA |
| 5 | pHW185-NP | pHW185-NP | pHW245-NP | pHW245-NP |
| 6 | pHW186-NA | pHW186-NA | pHW246-NA | pHW246-NA |
| 7 | pHW187-M | pHW187-M | pHW247-M | pHW247-M |
| 8 | pHW188-NS | pHW188-NS | pHW248-NS | pHW248-NS |
| Virus titer* | ||||
| 24 h | 0 | 1 × 103 | 0 | 0 |
| 48 h | 0 | 2 × 106† | 0 | 2 × 103 |
| 72 h | 0 | 2 × 107† | 0 | 2 × 105† |
*
The numbers represent infectious virus particles per milliliter of the supernatant of transfected cells as determined 24, 48, and 72 h after transfection.
†
Cytopathic effect in the cocultured MDCK cells was observed.