Figure 5 Denaturation of colonic epithelial cells (CEC)‐conditioned media (cond med) at 95°C for 10 min, 30 min and 2 h. The media were used for stimulation of CCD‐18Co cells for 24 h. As controls, cells were stimulated with unconditioned and non‐denatured, CEC‐conditioned media. Denaturation of the medium significantly (p<0.01) reduced interleukin 8 (IL8) secretion compared with CEC‐conditioned media, indicating a protein responsible for CLPF activation. Data are given as mean (SD). *p<0.01 versus Cond med CEC.