Figure 7 Immunoprecipitation of galectin‐3 from colonic epithelial cells (CEC)‐conditioned media. Protein‐G‐sepharose and anti‐human galectin‐3 antibody were added to the CEC‐conditioned medium and immunoprecipitated. The medium without anti‐galectin‐3 antibody was used as a control. The immunoprecipitated protein was analysed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. No bands were detectable in control medium. The CEC‐conditioned media showed one band at the molecular size of galectin‐3 at 30 kDa (additionally, the heavy chain of the galectin‐3 antibody was seen).