Abstract
A high-pressure liquid chromatographic method was developed for quantitative determination in human serum of a new penem antibiotic known as Sch 29482 (5R,6S,8R-2-ethylthio-6(1-hydroxyethyl)penem-3-carboxylic acid). The method involves serum extraction at an acid pH with ether, followed by separation on a reverse-phase column and UV light detection at 254 nm. This technique produced a good linear relationship between the peak height ratio and the Sch 29482 concentration, which ranged from 0.09 to 35.64 micrograms/ml. In addition, this procedure proved to be quite specific for Sch 29482, since all beta-lactam antibiotics tested did not interfere with the assay. For high concentrations (greater than 0.5 micrograms/ml), the mean values obtained from the high-pressure liquid chromatographic method correlated very well (r = 0.997) with those obtained from a microbiological assay. This method is accurate and reproducible, with a sensitivity of about 0.09 micrograms per ml of serum. It is useful for monitoring serum drug levels in animals and humans and can also be used for drug pharmacokinetic studies in humans.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Haughey D. B., Janicke D. M., Adelman M., Schentag J. J. High-pressure liquid chromatography analysis and single-dose disposition of tobramycin in human volunteers. Antimicrob Agents Chemother. 1980 Apr;17(4):649–653. doi: 10.1128/aac.17.4.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hekster Y. A., Baars A. M., Vree T. B., van Klingeren B., Rutgers A. Comparison of high performance liquid chromatography and microbiological assay in the determination of plasma cefuroxime concentrations in rabbits. J Antimicrob Chemother. 1980 Jan;6(1):65–71. doi: 10.1093/jac/6.1.65. [DOI] [PubMed] [Google Scholar]
- Nilsson-Ehle I., Yoshikawa T. T., Schotz M. C., Guze L. B. Quantitation of antibiotics by high-pressure liquid chromatography: cephalothin. Antimicrob Agents Chemother. 1978 Feb;13(2):221–227. doi: 10.1128/aac.13.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peng G. W., Jackson G. G., Chiou W. L. High-pressure liquid chromatographic assay of netilmicin in plasma. Antimicrob Agents Chemother. 1977 Dec;12(6):707–709. doi: 10.1128/aac.12.6.707. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki A., Noda K., Noguchi H. High-performance liquid chromatographic determination of ceftizoxime, a new cephalosporin antibiotic, in rat serum, bile and urine. J Chromatogr. 1980 Jun 13;182(3-4):448–453. doi: 10.1016/s0378-4347(00)81499-1. [DOI] [PubMed] [Google Scholar]
- Tsuji K. Fluorimetric determination of erythromycin and erythromycin ethylsuccinate in serum by a high-performance liquid chromatographic post-column, on-stream derivatization and extraction method. J Chromatogr. 1978 Oct 1;158:337–348. doi: 10.1016/s0021-9673(00)89978-1. [DOI] [PubMed] [Google Scholar]
- Vree T. B., Hekster Y. A., Baars A. M., Van der Kleijn E. Rapid determination of amoxycillin (clamoxyl) and ampicillin (penbritin) in body fluids of many by means of high-performance liquid chromatography. J Chromatogr. 1978 May 1;145(3):496–501. doi: 10.1016/s0378-4347(00)81384-5. [DOI] [PubMed] [Google Scholar]