Figure 2 Effect of zinc carnosine (ZnC) on wound healing as assessed by cell migration. Standard wounds were inflicted on monolayers of human colonic HT29 cells and degree of movement assessed in the presence or absence of various concentrations of ZnC. Negative control was Dulbecco's modified Eagle medium (DMEM) alone, positive control (10% fetal calf serum). Maximal stimulation by ZnC was seen at 100 μM, where p<0.01 versus negative control for all times after 4 h. Addition of equimolar amounts of bovine serum albumin or zinc sulphate did not increase the rate of migration (data not shown). Similar results were seen when ZnC was tested on the canine epithelial cell line MDCK (not shown).