Table 1.

Quantitation of the effects of GST–SYP on SV number, docked SV number, and CCV number during high-frequency stimulation

	Vesicles	Docked vesicles	Coated vesicles
GST-1	98.13  ± 12.3	14.11  ± 1.64	0.84  ± 0.06
GST-2	71.2  ± 5.29	7.96  ± 0.63	0.77  ± 0.06
SYP-1	34.58  ± 2.87	3.42  ± 0.4	2.33  ± 0.3
SYP-2	22.77  ± 3.48	3.27  ± 0.49	1.76  ± 0.2
Average GST	84.66  ± 8.79	11.03  ± 1.13	0.8  ± 0.06
Average SYP	28.67  ± 3.17	3.34  ± 0.44	2.45  ± 0.25

Vesicle numbers are the mean number of synaptic vesicles per μm², clustered at active zones (mean ± SEM). Docked vesicles are defined as those within 0.1 vesicle diameters of the plasma membrane at the active zone. Coated vesicle numbers are the mean number of CCV per μm². The GST-1 and SYP-1 synapses were stimulated 25 min after injection for 1 min continuously (50 Hz, 750-ms trains with a 1.5-s interval between trains), allowed to rest for 1 min, and then given one final train of action potentials before fixation. The GST-2 and SYP-2 synapses were stimulated for 3 min continuously 50 min after injection and then fixed. Average GST and average SYP are averages of the values from the two GST-injected and GST–SYP-injected synapses, respectively.