Figure 3 Topical nuclear factor‐κB decoy (NFκBD) treatment reduces the amount of activated nuclear factor‐κB (NF‐κB) in the colon. (A) Colons from trinitrobenzene sulphonic acid (TNBS)‐induced mice were analysed for tissue NF‐κB levels over an 8‐day period using an ELISA‐based TransAm assay. Time course data were graphed with trend line and assessed by regression analysis. (B) Electrophoretic mobility shift assay using 32P‐labelled probe and nuclear extracts prepared from the colons of mice of the indicated treatment groups at the day 7 time point. ODN, phosphorothioated NFκBD oligonucleotide.