Figure 3 Immunohistochemical analysis of glial derived neurotrophic factor family receptor (GFR)α3 in normal pancreas (A–C) and in chronic pancreatitis (D–F). In normal pancreatic tissue samples, immunostaining of GFRα3 was minimally present in the smooth muscle cells of arteries (see arrows in (A)) and moderate to strong in ductal cells (C) and in islets (inset (C)). In chronic pancreatitis, GFRα3 showed moderate immunoreactivity in the smooth muscle cells of arteries (D), in nerve fibres, in the cytoplasm of Schwann cells (D, E) and in tubular complexes (F and inset F). Magnification 200× in images A, B and E; 100× in images C, D and F; chromogen: DAB.