Table 1 Analysis of proteins in human meibomian gland secretions with stringent SEQUEST criteria.
| Accession No | Protein | Number of peptides | Samples |
|---|---|---|---|
| NP_067048.1 | basic proline rich protein | 1 | Q |
| AAC82528.1 | IgA α1 chain C region | 3 | Q |
| AAB59396.1 | IgA α2 chain C region | 2 | Q |
| AAO11861.1 | immunoglobulin kappa light chain variable region | 1 | Q |
| NP_001562.1 | interferon regulatory factor 3 | 5 | V, U |
| NP_006112.2 | keratin 1 | 33 | Q, U, V, W |
| NP_000414.2 | keratin 2 | 15 | W |
| NP_476429.1 | keratin 3 | 1 | W |
| AAH24292.1 | keratin 5 | 13 | W |
| NP_005545.1 | keratin 6 | 11 | U, W |
| CAA82315.1 | keratin 9 | 13 | U, W |
| NP_000412.2 | keratin 10 | 2 | Q, R, U, W, X |
| NP_005548.2 | keratin 16 | 1 | R |
| NP_009175.1 | lacrimal proline rich protein | 1 | Q, R, U |
| NP_150593.1 | lacritin precursor | 1 | R |
| AAH22347.1 | lactotransferrin (lactoferrin) | 3 | Q |
| AAH15822.1 | lactotransferrin precursor | 3 | Q |
| NP_002288.1 | lipocalin 1 | 4 | R |
| AAC63078.1 | lysozyme C precursor | 1 | Q |
| AAH66594.1 | similar to IgA C region | 2 | Q |
| XP_208403.1 | similar to lipocalin 1 precursor | 3 | Q, R |
| BAC87456.1 | unnamed protein product | 3 | Q |
Human meibomian gland secretion samples (that is, Q, R, U, V, W, X, and Y) were processed with µHPLC/MS/MS procedures and the resulting ion spectra were filtered with stringent SEQUEST criteria. These parameters, as previously reported,15 yield less than 1% false positives. The proteins are listed with their accession number, the number of peptides that matched, and the samples in which they were identified.