Table 2 Identification of proteins in human meibomian gland secretions with less stringent SEQUEST criteria.
| Accession No | Protein | Number of peptides | Samples |
|---|---|---|---|
| A41948 | αfetoprotein enhancer binding protein | 2 | U, W |
| NP_001105.1 | adenylate cyclase 7 | 1 | W |
| NP_002323.1 | α2‐macroglobulin receptor | 2 | X, Y |
| NP_114121.1 | CLLL8 protein | 1 | W |
| AAH15130.1 | cytochrome c | 2 | U, W |
| CAA32479.1 | dystrophin (AA 1‐3685)[MASS = 10954] | 2 | R, V |
| AAN86577.1 | EVC2 protein (Ellis‐van Creveid syndrome) | 2 | W, X |
| Q96RI1 | Farnesoid X activated receptor | 2 | Q, W |
| BAC03456.1 | FLJ00397 protein[MASS = 16499] | 1 | W |
| AAA58477.1 | fork head related protein[MASS = 36713] | 1 | Q |
| XP_297722.1 | hypothetical protein | 1 | W |
| NP_710154.1 | hypothetical protein FLJ31051 | 2 | Q, U |
| T00391 | hypothetical protein KIAA0612 | 1 | W |
| XP_120509.1 | hypothetical protein XP_120509.1 | 2 | U, W |
| XP_296373.1 | hypothetical protein XP_296373.1[MASS = 10954] | 1 | Q |
| XP_296857.1 | hypothetical protein XP_296857 | 1 | U |
| XP_299999.1 | hypothetical protein XP_299999 | 2 | Q, R |
| P01876 | immunoglobulin[MASS = 37654] | 1 | Q |
| XP_290781.1 | keratin 14 | 2 | W |
| NP_149442.1 | keratin associated protein 4–13 | 3 | W, X |
| BAA31632.2 | KIAA0657 protein[MASS = 135917] | 1 | Q |
| BAA74870.1 | KIAA0847 protein[MASS = 70370] | 1 | W |
| BAA92565.2 | KIAA1327 protein | 5 | Q, R, W |
| BAB47507.1 | KIAA1878 protein[MASS = 48921] | 2 | W |
| Q16787 | Laminin α3 chain precursor | 2 | Q |
| CAB51072.1 | MSX2‐interacting protein | 2 | W |
| AAK21295.1 | NM‐4 | 2 | W |
| NP_005798 | proteoglycan 4; megakaryote stimulating factor | 1 | R |
| BAC06129.1 | seven transmembrane helix receptor | 2 | R, W |
| XP_225182.1 | similar to AU RNA‐binding protein/enoyl‐coenzyme A hydratase | 2 | Q, W |
| XP_291583.1 | similar to BarH‐class homeodomain transcription | 1 | W |
| XP_215959.1 | similar to chromosome 20 open reading frame 86 | 2 | W |
| XP_236991.1 | similar to colon cancer antigen NY‐CO‐45 | 2 | W |
| XP_223599.1 | similar to fidgetin‐like 1 | 1 | W |
| XP_224742.1 | similar to GEM‐interacting protein | 6 | W, X, Y |
| XP_208073.1 | similar to H3 histone, family 3B | 2 | W |
| XP_222620.1 | similar to hypothetical protein DKFZp434B1727 | 2 | R, W |
| XP_228642.1 | similar to hypothetical protein FLJ32191 | 2 | Q, W |
| XP_230513.1 | similar to hypothetical protein MGC14161 | 1 | W |
| XP_215716.1 | similar to hypothetical protein MGC27034 | 1 | W |
| XP_066017.1 | similar to keratin 18 | 1 | U |
| XP_139603.1 | similar to keratin 6 irs4 | 1 | W |
| XP_237187.1 | similar to KIAA0540 protein | 1 | W |
| XP_218841.1 | similar to KIAA1233 protein | 2 | X |
| XP_027915.7 | similar to KIAA1405 protein | 3 | U, W |
| XP_233885.1 | similar to KIAA1805 protein | 6 | Q, U, W, Y |
| XP_219779.1 | similar to KIAA2026 protein | 2 | R, W |
| XP_297211.1 | similar to ladinin 1 | 1 | R, W |
| XP_026998.2 | similar to leucine rich repeat‐containing 8 | 1 | U |
| XP_071099.5 | similar to olfactory receptor MOR262‐2 | 1 | Q |
| XP_290771.1 | similar to RIKEN cDNA 3110023B02 | 2 | Q, R |
| XP_232995.1 | similar to ring finger protein 20 | 1 | W |
| XP_236770.1 | similar to topoisomerase (DNA) II binding protein | 1 | W |
| XP_231546.1 | similar to zinc finger, imprinted 3 | 2 | W |
| NP_477521.1 | small breast epithelial mucin | 1 | R |
| NP_004644.1 | SmcY protein (histocompatibility Y antigen) | 3 | W, Y |
| NP_710161.1 | synaptonemal complex protein 3 | 2 | R, W |
| NP_115986.2 | testes developmental related NYD‐SP21 | 1 | W |
| NP_004771.1 | transcription elongation factor A (SII)‐like 1 | 1 | U |
| I73633 | TrkC tyrosine kinase | 1 | W |
| 0501254A | Tro α1 H, myeloma | 1 | Q |
| NP_003106.2 | UDP‐N‐acetylglucosamine pyrophosphorylase 1 | 1 | W |
| BAB14689.1 | unnamed protein product[MASS = 148993] | 1 | W |
| BAB70833.1 | unnamed protein product[MASS = 32306] | 2 | R, W |
| BAC05042.1 | unnamed protein product[MASS = 80996] | 2 | R, W |
| O75844 | zinc metalloproteinase | 1 | W |
Proteins in human meibomian gland secretion samples (that is, Q, R, U, V, W, X, and Y) were analysed using µHPLC/MS/MS techniques and broad SEQUEST criteria (that is, Xcorr >1.3, ΔCn >0.8). To decrease the possibility of false positives, all MS data were manually confirmed. If proteins were already identified with more stringent criteria (that is, table 1), they are not listed again in this table. The column headings are analogous to those in table 1.