Table 3.

Real-time PCR analysis of adrenal gene expression: verification of selected microarray results.

		Hypoxia vs. Normoxia (food ad libitum)	Mean Delta Ct
Gene	Symbol	Percent of Normoxia	Normoxia	Hypoxia
Cytochrome P450, subfamily 1B, polypeptide 1	Cyp1b1	197^*	13.53 ± 0.15	12.55 ± 0.04
Guanosine monophosphate reductase	Gmpr	67 ¹	15.73 ± 0.20	16.34 ± 0.14
Xanthine dehydrogenase	Xdh	137	19.77 ± 0.16	19.33 ± 0.22
Cadherin 1	Cdh1	62	19.58 ± 0.34	20.27 ± 0.31
Phospholipase A2, group IVA (cytosolic)	Pla2g4a	73	18.85 ± 0.28	19.32 ± 0.09
Aldehyde oxidase	Aox1	84	18.94 ± 0.12	19.19 ± 0.28
Regulator of G-protein signaling protein 2	Rgs2	100 ¹	16.16 ± 0.11	16.14 ± 0.11
Carnitine palmitoyltransferase 2	Cpt2	94 ¹	12.76 ± 0.05	12.84 ± 0.11
Steroid 5 alpha-reductase 1	Srd5a1	130	15.29 ± 0.16	14.91 ± 0.12
Enolase 1, alpha	Eno1	135	11.63 ± 0.12	11.20 ± 0.16
N-ethylmaleimide sensitive factor	Nsf	130^*	18.03 ± 0.06	17.65 ± 0.11
Cytochrome P450, subfamily 11B, polypeptide 3	Cyp11b3	67	9.75 ± 0.14	10.30 ± 0.25

Whole adrenal glands were pooled (24 glands or one litter/sample) and total RNA was extracted according to the manufacturer's protocol. Genes picked for verification with real-time PCR were chosen based on their possible significance in adrenal function. Real-time PCR results that did not agree with microarray results were re-assayed. Data are presented as percent of control (Normoxia).

Significant difference from Normoxia with p<0.05.

Result did not agree with microarray result (see Discussion). N=4 per treatment group.

Note: an increase in the Delta Ct value represents a decrease in mRNA expression.