Table 4.

Real-time PCR analysis of adrenal gene expression: analysis of genes not meeting microarray cutoff criteria.

					Mean Delta Ct
Gene	Symbol	Hypoxia vs. Normoxia (food ad libitum)	Normoxia Pair Fed vs. Normoxia (food ad libitum)	Hypoxia vs Normoxia Pair Fed	Normoxia (ad libitum)	Hypoxia	Normoxia Pair Fed
Cytochrome P450, subfamily 1B, polypeptide 1	Cyp1b1	158*	50	313*	11.52 ± 0.28	10.87 ± 0.32	12.51 ± 0.21
Steroidogenic acute regulatory protein	StAR	148	60*	244*	8.29 ± 0.05	7.72 ± 0.21	9.02 ± 0.29
Cytochrome P450, subfamily 11A	Cyp11a	117	72*	164	9.09 ± 0.14	8.86 ± 0.18	9.57 ± 0.17
Cytochrome P450, subfamily 21A, polypeptide 1	Cyp21a1	139*	67*	208*	9.55 ± 0.09	9.07 ± 0.02	10.12 ± 0.12
Low density lipoprotein receptor	Ldlr	153	58	233*	20.24 ± 0.16	19.82 ± 0.21	21.03 ± 0.30
Vascular endothelial growth factor	Vegf	117	86	135	15.74 ± 0.03	15.52 ± 0.16	15.95 ± 0.10
Hypoxia-inducible factor 1 alpha	Hif1a	100	83	120	12.59 ± 0.08	12.58 ± 0.19	12.86 ± 0.15

Whole adrenal glands were pooled (24 glands/sample) and total RNA was extracted according to the manufacturer's protocol. Data are presented as percent of control (Normoxia or Normoxia Pair Fed). Genes assayed for these real-time PCR analyses were chosen based on their known roles in the steroidogenic pathway or in the cellular response to hypoxia.

^*Significant difference from Normoxia or Normoxia Pair Fed with p<0.05. N=4 per treatment group.

Note: an increase in the Delta Ct value represents a decrease in mRNA expression.