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. Author manuscript; available in PMC: 2008 Jan 15.
Published in final edited form as: Arch Biochem Biophys. 2006 Nov 17;457(2):177–186. doi: 10.1016/j.abb.2006.11.006

Figure 2. Concentration dependent digestion of collagen I by MMP-1 and APCE or rFAP.

Figure 2

Rat tail collagen type I was gelled at 2 mg/ml and incubated at 37 °C for 24 hours with enzymes in 12.5 mM Na PO4 buffer, pH 7.5. Panel A. Lane: 1) 1.5 μg MMP-1; 2) 1.5 μg MMP-1, 2 μg APCE; 3) 1.5 μg MMP-1, 4 μg APCE; 4) 1.5 μg MMP-1, 8 μg APCE; 5) 1.5 μg MMP-1, 12 μg APCE; 6) 1.5 μg MMP-1, 16 μg APCE; 7) 16 μg APCE. Panel B. Lane: 1) 1.5 μg MMP-1; 2) 1.5 μg MMP-1, 2 μg rFAP; 3) 1.5 μg MMP-1, 4 μg rFAP; 4) 1.5 μg MMP-1, 8 μg rFAP; 5) 1.5 μg MMP-1, 12 μg rFAP; 6) 1.5 μg MMP-1, 16 μg rFAP; 7) 16 μg rFAP. Digests were reduced and analyzed by 4–12% SDS-PAGE. Where present, the ~97 kDa band is either APCE or rFAP.