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. Author manuscript; available in PMC: 2008 Jan 15.
Published in final edited form as: Arch Biochem Biophys. 2006 Nov 17;457(2):177–186. doi: 10.1016/j.abb.2006.11.006

Figure 4. Liquid chromatography of peptides produced from collagen I digestion.

Figure 4

Rat tail collagen type I, gelled at 2 mg/ml was incubated at 37 °C for 24 hours with 1.5 μg MMP-1 (black), 1.5 μg MMP-1, 15 μg APCE (green) or 1.5 μg MMP-1, 20 mM EDTA, 15 μg APCE (red). Samples were precipitated with 70% acetonitrile and the supernatant analyzed by reverse phase chromatography.