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. Author manuscript; available in PMC: 2007 Sep 1.
Published in final edited form as: Exp Eye Res. 2007 Jan 10;84(3):513–528. doi: 10.1016/j.exer.2006.11.004

Fig. 10.

Fig. 10

Effects of expression of the Cx50 transgene on solubility of crystallins. Immunoblots of total homogenates (lanes 1 and 2), soluble fractions (lanes 3 and 4) and insoluble fractions (lanes 5 and 6) prepared from non-transgenic (lanes 1, 3 and 5) and transgenic (lanes 2, 4 and 6) mouse lenses from line 5 using anti-αA-, anti-αB-, anti-β- or anti-γ-crystallin (A–D, respectively) antibodies. Samples from 6-month-old and 4-month-old mice were used for immunoblots of αA- and αB-crystallin and β- and γ-crystallin, respectively. No major differences in the levels of the studied crystallins or their distribution between soluble and insoluble fractions were detected between non-transgenic and transgenic lenses.