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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: J Mol Cell Cardiol. 2006 Dec 21;42(3):590–599. doi: 10.1016/j.yjmcc.2006.12.007

Figure 6.

Figure 6

Phosphatase 1 appears to be the major phosphatase dephosphorylating CaMKII in cardiomyocytes. Pacing triggers autophosphorylation of CaMKII only when phosphatase 1 (and 2a) is inhibited, but not when phosphatase 2b (calcineurin) or 2a alone are inhibited. Phosphatase inhibitors used were cypermethrin (1 μM; 30 min preincubation), okadaic acid (200 nM; 30 min precincubation) and calyculin A (1 μM; 10 min precincubation). Phosphatase 2b was also inhibited by cyclosporin A, phosphatase 2a by cantharidin and phosphatase 1 by higher concentrations of OA yielding the same result (data not shown).