Fig. 2.
Trimer formation by cleaved, wild-type and mutant KNH1144 gp140 proteins. (A) SOS and SOSIP versions of KNH1144 gp140 proteins. (B) KNH1144 SOSIP gp140 mutants containing the indicated single residue substitutions in the gp41 N-terminal region, compared with the wild-type KNH1144 SOSIP gp140. (C) KNH1144 SOSIP and SOS mutant gp140s, as indicated. Each panel shows a BN-PAGE analysis, followed by western blotting using MAb CA13.