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. Author manuscript; available in PMC: 2007 Oct 1.
Published in final edited form as: Protein Expr Purif. 2006 Dec 1;52(2):334–339. doi: 10.1016/j.pep.2006.11.009

Fig. 1.

Fig. 1

Purification of recombinant, C-terminally tagged, NS2B-NS3pro and NS2B-NS3 K48A constructs on a Co2+-chelating Sepharose Fast Flow column (A and B, respectively). The left panels show the profile of elution of the constructs. To remove impurities, the protease was eluted with 100 ml of 10–500 mM gradient of imidazole (100 ml) at a 0.5 ml/min flow rate. Fractions containing the protease are indicated by a bracket. The middle and the right panels show the gel electrophoresis analysis of the fractions eluted from the column during chromatography and re-chromatography of the protease samples, respectively.