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. 2007 Jan 29;176(3):319–328. doi: 10.1083/jcb.200604106

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — Effect of CDC37 mutation on protein kinase levels. (A) Schematic of the CDC37 locus showing the position where URA3 was inserted as well as the serine 14 to alanine mutation (S14A) in CDC37. Arrows denote the direction of transcription. (B) Four examples of unstable kinases identified with anti-TAP (cells grown at 30°C). Western blots of the same samples with anti-Pgk1 are shown as loading controls. (C) Four examples of kinases that were unchanged after culturing wild type and cdc37^S14A at 30°C. (D) Phylogenetic analysis of protein kinases used in this study. Kinases that accumulated to wild-type levels in the cdc37^S14A mutant are shown in red. Kinases in black accumulated at less than twofold compared with wild-type levels.