Figure 1.
Direct activation of icap by 12-(S)-HPETE, one of LO products. (A) 12-(S)-HPETE in 10 μM activates single-channel currents in an inside-out membrane patch of a cultured dorsal root ganglion neuron containing capsaicin (CAP)-activated channels. (Inset) Current traces in an expanded time scale. (B) Block by 10 μM capsazepine (CZP) of single channel currents activated by 2 μM 12-(S)-HPETE. (Insets) A summary of the block by capsazepine (Left). Channel current is expressed in channel activity (NPo). **, P < 0.01. (Right) Current-voltage relationship of channels activated by capsaicin (open circle, n = 11) and 12-(S)-HPETE (filled triangle, n = 5–8). The two I–V curves are superimposable. Concentrations are expressed in micromolar.