Skip to main content
. 2000 May 16;97(11):6161–6166. doi: 10.1073/pnas.100121097

Figure 1.

Figure 1

Apoptosis in cerebrocortical cultures. Cultures were exposed to either 300 μM NMDA/5 μM glycine ± 1 μM BA, or 0.5 μM staurosporine. (a–c) Representative labeling of apoptotic cells with PI, (d–f) with SYTO-13, or (g–i) by ISEL+. (j) At 18 h post insult, the presence of cells with condensed nuclei was assessed by staining with either PI (open bars) or SYTO-13 (shaded bars). DNA strand breaks within individual cells were evaluated at 18 h by ISEL+ (filled bars). Values are corrected for the basal level of apoptosis in these cultures (11.7 ± 2.1%; mean ± SEM). Statistical significance of BA/NMDA compared with NMDA indicated by asterisks (**, P < 0.001; ***, P < 0.0001). (k) Protection produced by BA is concentration dependent. Cells treated with NMDA/glycine ± BA (●) were analyzed for apoptosis at 18 h using PI. Treatment of cultures with BA alone for 30 min did not result in an increase in apoptosis at 18 h (□). BA (0.1 μM) did not protect from NMDA-induced apoptosis (***, P < 0.001; n = 8).