Table 2 Antigens studied.
| Antigen | Target | Antibody source | Antigen retrieval | Antibody dilution |
|---|---|---|---|---|
| CD45RO | T lymphocytes | Dako | Heat mediated | 1:800 |
| CD45 | T lymphocytes | Dako | Heat mediated | 1:800 |
| CD20cy | B lymphocytes | Dako | Heat mediated | 1:600 |
| Mac 387 | Macrophages | Dako | Trypsin | 1:100 |
| CD68 | Macrophages | Dako | Trypsin | 1:100 |
| Cam 5.2 | RPE cells | BD Biosciences | Trypsin | 1:50 |
| GFAP | Glial tissue | Dako | Trypsin | 1:2000 |
GFAP, glial fibrillary acidic protein; RPE, retinal pigment epithelium.
Antigen retrieval‐tissue sections were treated by either:(1) heat mediation, performed in an 800 W microwave oven by heating in 50 g/l urea in 50 mM TRIS‐HCl buffer pH 9.5 for 10 min followed by cooling for a further 25 min Or (2) trypsin, performed in a 37°C incubator by exposure to 1 g/l trypsin in 100 mm TRIS‐HCl buffer pH 7.8 for 15 min.
Dako, Ely, UK; BD Biosciences New Jersey, USA.