Figure 5 The effect of tacrolimus on tumour necrosis factor (TNF)α levels in the supernatant derived from lipopolysaccharide stimulated macrophages. Peritoneal exudates cells treated with vehicle or tacrolimus were with LPS (0.1 μg/ml) for 24 h. The protein level of TNFα was measured by ELISA. Data are the mean±SEM (n = 3). The experiment was repeated twice with similar results.