Figure 3 Immunocytochemical analysis of NF‐κB p65 localisation in HLE B‐3 cells after treatment with H₂O₂ in the absence or presence of PDTC. A: In untreated cells, NF‐κB p65 primarily existed in the cytoplasm, not in the nuclei. B: After stimulated with 0.5 mM of H₂O₂ for 1 hour, NF‐κB p65 translocated from the cytoplasm into the nuclei. C: In the cells pretreated with 100 μM of PDTC for 2 hours, few NF‐κB p65 translocated into the nucleus.