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. 2007 Apr 27;3(4):e60. doi: 10.1371/journal.ppat.0030060

Figure 6. The IL-6 Response to E. coli of BECs Is the Product of Two Separate Signaling Pathways.

Figure 6

(A) IL-6 secretion response to E. coli ORN103(pSH2) by WT and AC-3 KD BECs in the absence or presence of pyrrolidine dithiocarbamate (PDTC).

(B) IL-6 secretion response to UPEC CI5 by WT and AC-3 KD BECs.

(C and D) IL-6 secretion responses by Mono Mac 6 cell line (C) and 16-HBE cell line (D). Cells were treated for 12 h with E. coli ORN103(pSH2) in the absence (EC) or presence of NiCl2 or PKA inhibitor (PKI). *, p < 0.03 relative to untreated (UT) value. Identical results were obtained after 6 h of treatment with E. coli ORN103(pSH2).

(E) Increased CREB phosphorylation in BECs in response to TLR2 and TLR3 ligands. Western blotting for phospho-CREB and total-CREB in UT BECs or BECs treated with either a TLR2 ligand (lipoteichoic acid) or a TLR3 ligand (polyinosine-polycytidylic acid).