Figure 2.

NPY mRNA and peptide levels in MBH (Left) and POA (Right) of juvenile and pubertal agonadal male monkeys. (a) Autoradiograms of NPY and Cyclo mRNAs hybridized to ³²P-labeled antisense probes generated from rat cDNAs. Use of an antisense probe transcribed from hNPY cDNA in additional RNase protection assays of pools containing equal amounts of RNA from each hypothalamic region yielded the same result (not shown). (b) Corresponding relative OD (mean ± SD) of the NPY mRNAs in juvenile (open bar, n = 4) and pubertal monkeys (filled bar, n = 4). (c) NPY peptide content (mean ± SD, n = 4 in each group). *, P < 0.05.